论文题目：Copy number variation of a gene cluster encoding endopolygalacturonase mediates flesh texture and stone adhesion in peach
作 者：Chao Gu1, Lu Wang1, Wei Wang1, Hui Zhou1,2, Baiquan Ma1,2, Hongyu Zheng1,2, Ting Fang1,2, Collins Ogutu1,2, Sornkanok Vimolmangkang1,3 and Yuepeng Han1,4
参与单位：1 Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Sino-African Joint Research Center, Wuhan Botanical Garden of the Chinese Academy of Sciences, Wuhan 430074, China
2 Graduate University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, China
3 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
4 College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong 271018, China
摘 要：Texture is an important attribute affecting consumer perception of fruit quality. Peach melting flesh and flesh adhesionto stone (endocarp) are simply inherited and controlled by the F-M locus on linkage group (LG) 4. Here, we report that two genes encoding endopolygalacturonase (endoPG) in the F-M locus, designated PpendoPGF and PpendoPGM, are associated with the melting flesh and stone adhesion traits. PpendoPGM controls melting flesh while PpendoPGF has pleiotropic effects on both melting flesh and stone adhesion. The F-M locus has three allelic copy number variants of endoPG, H1 (PpendoPGF and PpendoPGM), H2 (PpendoPGM), and H3 (null). The H2 haplotype represents the ancestral one while the H1 and H3 haplotypes are two variants due to duplication and deletion of PpendoPGM, respectively. Accessions with H1H1, H1H2, or H1H3 genotypes show the freestone or semi-freestone and melting flesh phenotype, while both H2H2 and H2H3 accessions have the clingstone and melting flesh phenotype. The H3H3 accessions have the clingstone and non-melting flesh phenotype. Our study not only demonstrates a driving role of gene copy number variations in flesh texture diversification in fruit trees, but also provides a useful diagnostic tool for early seedling selection in peach breeding programmes.
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