论文题目：Evolutionary origin of Rosaceae-specific active non-autonomous hAT elements and their contribution to gene regulation and genomic structural variation
作 者：Lu Wang1* Qian Peng1,3* Jianbo Zhao2* Fei Ren2* Hui Zhou1,3*Wei Wang1* Liao Liao1,4* Albert Owiti1,3* Quan Jiang2* Yuepeng Han1,4
参与单位：1 Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences, Wuhan 430074, People’s Republic of China
2 Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, A12, Ruiwangfen, Beijing 100093, People’s Republic of China
3 Graduate University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, People’s Republic of China
4 Sino-African Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, People’s Republic of China
摘 要：Transposable elements account for approximately 30 % of the Prunus genome; however, their evolutionary origin and functionality remain largely unclear. In this study, we identified a hAT transposon family, termed Moshan, in Prunus. The Moshan elements consist of three types, aMoshan, tMoshan, and mMoshan. The aMoshan and tMoshan types contain intact or truncated transposase genes, respectively, while the mMoshan type is miniature inverted-repeat transposable element (MITE). The Moshan transposons are unique to Rosaceae, and the copy numbers of different Moshan types are significantly correlated. Sequence homology analysis reveals that the mMoshan MITEs are direct deletion derivatives of the tMoshan progenitors, and one kind of mMoshan containing a MuDR-derived fragment were amplified predominately inthe peach genome. The mMoshan sequences contain cisregulatory elements that can enhance gene expression up to 100-fold. The mMoshan MITEs can serve as potential sources of micro and long noncoding RNAs. Who e-genome re-sequencing analysis indicates that mMoshan elements are highly active, and an insertion into S-haplotypespecific F-box gene was reported to cause the breakdown of self-incompatibility in sour cherry. Taken together, all these results suggest that the mMoshan elements playimportant roles in regulating gene expression and driving genomic structural variation in Prunus.
